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Orgel, L, A'Hearn M, Bada J, Baross J, Chapman C, Drake M, Kerridge J, Race M, Sogin M, Squyres S.  2000.  Sample return from small solar system bodies. Advances in Space Research. 25:239-48.   10.1016/s0273-1177(99)00954-0   AbstractWebsite

With plans for multiple sample return missions in the next decade, NASA requested guidance from the National Research Council's Space Studies Board on how to treat samples returned from solar system bodies such as planetary satellites, asteroids and comets. A special task group assessed the potential for a living entity to be included in return samples from various bodies as well as the potential for large scale effects if such an entity were inadvertently introduced into the Earth's biosphere. The group also assessed differences among solar system bodies, identified investigations that could reduce uncertainty about the bodies, and considered risks of returned samples compared to the natural influx of material to the Earth in the form of interplanetary dust particles, meteorites and other small impactors. The final report (NRC, 1998) provides a decision making framework for future missions and makes recommendations on how to handle samples from different planetary satellites and primitive solar system bodies

Onstott, TC, Magnabosco C, Aubrey AD, Burton AS, Dworkin JP, Elsila JE, Grunsfeld S, Cao BH, Hein JE, Glavin DP, Kieft TL, Silver BJ, Phelps TJ, van Heerden E, Opperman DJ, Bada JL.  2014.  Does aspartic acid racemization constrain the depth limit of the subsurface biosphere? Geobiology. 12:1-19.   10.1111/gbi.12069   AbstractWebsite

Previous studies of the subsurface biosphere have deduced average cellular doubling times of hundreds to thousands of years based upon geochemical models. We have directly constrained the in situ average cellular protein turnover or doubling times for metabolically active micro-organisms based on cellular amino acid abundances, D/L values of cellular aspartic acid, and the in vivo aspartic acid racemization rate. Application of this method to planktonic microbial communities collected from deep fractures in South Africa yielded maximum cellular amino acid turnover times of similar to 89years for 1km depth and 27 degrees C and 1-2years for 3km depth and 54 degrees C. The latter turnover times are much shorter than previously estimated cellular turnover times based upon geochemical arguments. The aspartic acid racemization rate at higher temperatures yields cellular protein doubling times that are consistent with the survival times of hyperthermophilic strains and predicts that at temperatures of 85 degrees C, cells must replace proteins every couple of days to maintain enzymatic activity. Such a high maintenance requirement may be the principal limit on the abundance of living micro-organisms in the deep, hot subsurface biosphere, as well as a potential limit on their activity. The measurement of the D/L of aspartic acid in biological samples is a potentially powerful tool for deep, fractured continental and oceanic crustal settings where geochemical models of carbon turnover times are poorly constrained. Experimental observations on the racemization rates of aspartic acid in living thermophiles and hyperthermophiles could test this hypothesis. The development of corrections for cell wall peptides and spores will be required, however, to improve the accuracy of these estimates for environmental samples.