Direct isolation of purines and pyrimidines from nucleic acids using sublimation

Citation:
Glavin, DP, Schubert M, Bada JL.  2002.  Direct isolation of purines and pyrimidines from nucleic acids using sublimation. Analytical Chemistry. 74:6408-6412.

Date Published:

Dec

Keywords:

amino-acids, bases, cytosine, deoxyribonucleic-acid, enthalpies, pyrolysis

Abstract:

A sublimation technique was developed to isolate purines and pyrimidines directly from lambda-deoxyribonucleic acid (lambda-DNA) and Escherichia coli cells. The sublimation of adenine, cytosine, guanine, and thymine from lambda-DNA was tested under reduced pressure (similar to 0.5 Torr) at temperatures of > 150 degreesC. With the exception of guanine, approximately 60-75% of each base was sublimed directly from the lambda-DNA and recovered on a coldfinger of the sublimation apparatus after heating to 450 T. Several nucleobases including adenine, cytosine, thymine, and uracil were also recovered from E. coli bacteria after heating the cells to the same temperature, although some thermal decomposition of the bases also occurred. These results demonstrate the feasibility of using sublimation to isolate purines and pyrimidines from native E. coli DNA and RNA without any chemical treatment of the cells.

Notes:

n/a

Website

DOI:

10.1021/ac0259663