Detection of the free-living forms of sulfide-oxidizing gill endosymbionts in the lucinid habitat (<i>Thalassia testudinum</i> environment)

Citation:
Gros, O, Liberge M, Heddi A, Khatchadourian C, Felbeck H.  2003.  Detection of the free-living forms of sulfide-oxidizing gill endosymbionts in the lucinid habitat (Thalassia testudinum environment). Applied and Environmental Microbiology. 69:6264-6267.

Date Published:

Oct

Keywords:

16s ribosomal-rna, aposymbiotic juveniles, bacterial, codakia-orbicularis bivalvia, endosymbionts, in-situ hybridization, oligonucleotide probes, sea hydrothermal vents, symbiotic bacteria, vertical transmission, weevil sitophilus-oryzae

Abstract:

Target DNA from the uncultivable Codakia orbicularis endosymbiont was PCR amplified from sea-grass sediment. To confirm that such amplifications originated from intact bacterial cells rather than free DNA, whole-cell hybridization (fluorescence in situ hybridization technique) with the specific probe Symco2 was performed along with experimental infection of aposymbiotic juveniles placed in contact with the same sediment. Taken together, the data demonstrate that the sulfide-oxidizing gill endosymbiont of Codakia orbicularis is present in the environment as a free-living uncultivable form.

Notes:

n/a

Website

DOI:

10.1128/aem.69.10.6264-6267.2003